SRA

The human blood-brain barrier (hBBB) is a highly specialized structure that regulates passage across blood and CNS compartments. Despite its critical physiological role, there are no reliable in vitro models that can mimic hBBB development and function. Here, we constructed hBBB assembloids from brain and blood vessel organoids derived from human pluripotent stem cells. We validated the acquisition of BBB-specific molecular, cellular, transcriptomic, and functional characteristics and uncovered an extensive neuro-vascular crosstalk with a spatial pattern within hBBB assembloids. When we used patient-derived hBBB assembloids to model cerebral cavernous malformations (CCMs), we found that these assembloids recapitulated the cavernoma anatomy and BBB breakdown observed in patients. Upon comparison of phenotypes and transcriptome between patient-derived hBBB assembloids and primary human cavernomas tissues, we uncovered CCM-related molecular and cellular alterations. Taken together, we report hBBB assembloids that mimic core properties of the human BBB and identify a potentially underlying cause of CCMs. Overall design: BBB assembloids was prepared by combining a H9 ESCs derived cerebral organoids, where the astrocytic differentiation is promotated by adding LIF with 15%FBS, with a blood vessel organoid from H9 ESCs indurced mesoderm. Both organoids were developed separately and embedded in a matrigel droplet. We then repurposed the used Illumina Nextseq 2000 sequencing flow cells to a spatial barcoded matrix for RNA hybirdizations. By appling the HIFI-Slide protocol on the assembloid fresh frozen sections for RNA capture and library preparations, we sequencing the extracted library to confirm the cell type and gene expression co-localizations of the BBB assembloids.